GT RT-qPCR SARS-CoV-2 Variants of Concern Mutational Signature Assay Kit
200 Tests. Kit requires 3 reactions to be run for each sample. This assay contains all primers, probes, and controls for detection of the signature mutations within the Spike protein-gene of the Alpha (UK, B.1.1.7), Beta (South African, B.1.351), Gamma (Brazilian/Japanese, P.1), Delta (Indian, B.1.617.2), and Epsilon (Californian, B.1.427/B.1.429) variants. While this test does not target every mutation present in these virus variants, it targets variant-associated genomic sequences corresponding to Spike protein amino acids 69-70 and 501 (reaction 1), 501, 417, and 484 (reaction 2), and 452 and 478 (reaction 3). The first reaction provides detection of the signature mutations associated with the Alpha (N501Y and del69-70) variant. The second reaction provides detection of the signature mutations associated with the Beta (E484K and K417N) and Gamma (E484K and K417T) variants. The third reaction provides detection of the signature mutations associated with the Delta (L452R and T478K) variant. Previous reports suggest these residues impact protein folding, and amino acid substitutions at these residues alter ACE-2 receptor binding and potentially promote immune system evasion. Kit includes validated GT-2-plex (501Y, del69-70) primer probe solution, GT-4-plex (N501Y, E484K, K417T, K417N) primer probe solution, GT-2-plex (L452R, T478K) primer probe solution, plus validated, quantitative assay standards: GT-Parental (Wuhan) standard, GT-Alpha (UK) Standard, GT-Beta (South African) Standard, GT-Gamma (Brazilian/Japanese) Standard, GT-Delta (Indian) Standard, and the GT-Epsilon (Californian) Standard.
SKU: 100180
200 Tests. Kit requires 3 reactions to be run for each sample. This assay contains all primers, probes, and controls for detection of the signature mutations within the Spike protein-gene of the Alpha (UK, B.1.1.7), Beta (South African, B.1.351), Gamma (Brazilian/Japanese, P.1), Delta (Indian, B.1.617.2), and Epsilon (Californian, B.1.427/B.1.429) variants. While this test does not target every mutation present in these virus variants, it targets variant-associated genomic sequences corresponding to Spike protein amino acids 69-70 and 501 (reaction 1), 501, 417, and 484 (reaction 2), and 452 and 478 (reaction 3). The first reaction provides detection of the signature mutations associated with the Alpha (N501Y and del69-70) variant. The second reaction provides detection of the signature mutations associated with the Beta (E484K and K417N) and Gamma (E484K and K417T) variants. The third reaction provides detection of the signature mutations associated with the Delta (L452R and T478K) variant. Previous reports suggest these residues impact protein folding, and amino acid substitutions at these residues alter ACE-2 receptor binding and potentially promote immune system evasion. Kit includes validated GT-2-plex (501Y, del69-70) primer probe solution, GT-4-plex (N501Y, E484K, K417T, K417N) primer probe solution, GT-2-plex (L452R, T478K) primer probe solution, plus validated, quantitative assay standards: GT-Parental (Wuhan) standard, GT-Alpha (UK) Standard, GT-Beta (South African) Standard, GT-Gamma (Brazilian/Japanese) Standard, GT-Delta (Indian) Standard, and the GT-Epsilon (Californian) Standard.
SKU: 100180
200 Tests. Kit requires 3 reactions to be run for each sample. This assay contains all primers, probes, and controls for detection of the signature mutations within the Spike protein-gene of the Alpha (UK, B.1.1.7), Beta (South African, B.1.351), Gamma (Brazilian/Japanese, P.1), Delta (Indian, B.1.617.2), and Epsilon (Californian, B.1.427/B.1.429) variants. While this test does not target every mutation present in these virus variants, it targets variant-associated genomic sequences corresponding to Spike protein amino acids 69-70 and 501 (reaction 1), 501, 417, and 484 (reaction 2), and 452 and 478 (reaction 3). The first reaction provides detection of the signature mutations associated with the Alpha (N501Y and del69-70) variant. The second reaction provides detection of the signature mutations associated with the Beta (E484K and K417N) and Gamma (E484K and K417T) variants. The third reaction provides detection of the signature mutations associated with the Delta (L452R and T478K) variant. Previous reports suggest these residues impact protein folding, and amino acid substitutions at these residues alter ACE-2 receptor binding and potentially promote immune system evasion. Kit includes validated GT-2-plex (501Y, del69-70) primer probe solution, GT-4-plex (N501Y, E484K, K417T, K417N) primer probe solution, GT-2-plex (L452R, T478K) primer probe solution, plus validated, quantitative assay standards: GT-Parental (Wuhan) standard, GT-Alpha (UK) Standard, GT-Beta (South African) Standard, GT-Gamma (Brazilian/Japanese) Standard, GT-Delta (Indian) Standard, and the GT-Epsilon (Californian) Standard.
SKU: 100180